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Related Concept Videos

Ion Exchange01:17

Ion Exchange

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Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
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In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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A polyethyleneimine-functionalized polymer substrate polar stationary phase.

Yufeng Shen1, Huiliang Geng1, Feifang Zhang1

  • 1Engineering Research Center of Pharmaceutical Process Chemistry, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East-China University of Science and Technology, 130 Meilong RD, Pharmacy School, Shanghai 200237, China.

Journal of Chromatography. A
|December 31, 2022
PubMed
Summary

A novel polyethyleneimine (PEI)-functionalized stationary phase for hydrophilic interaction chromatography (HILIC) offers unique selectivity for polar analytes. This robust HILIC phase demonstrates excellent stability across a wide pH range and low column bleed.

Keywords:
BleedHydrophilic interaction liquid chromatographyPolyethyleneiminePolymerStationary phase

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Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Materials Science

Background:

  • Hydrophilic interaction chromatography (HILIC) is crucial for separating polar compounds.
  • Developing robust and selective stationary phases remains a key challenge in HILIC.
  • Polymer-supported stationary phases offer tunable properties for chromatographic applications.

Purpose of the Study:

  • To synthesize and characterize a novel polyethyleneimine (PEI)-functionalized stationary phase for HILIC.
  • To investigate the retention mechanisms and selectivity of the new PEI-functionalized phase.
  • To evaluate the column stability and performance under various chromatographic conditions.

Main Methods:

  • Grafting PEI onto poly(styrene-divinylbenzene) (PS-DVB) microspheres to create the stationary phase.
  • Utilizing hydrophilic interaction chromatography (HILIC) for analyte separation.
  • Employing gradient elution and analyzing retention profiles for polar, acidic, basic, and neutral compounds.

Main Results:

  • The PEI-functionalized phase exhibited a U-shaped retention profile, characteristic of HILIC with >60% organic solvent.
  • Retention mechanisms involved hydrogen bonding, anion exchange, and hydrophobic interactions.
  • Achieved excellent separation and unique selectivity for diverse polar analytes, with extremely low column bleed and wide pH tolerance (1-13).

Conclusions:

  • The developed PEI-functionalized stationary phase is a promising material for HILIC applications.
  • Its unique selectivity and robust performance make it suitable for complex sample analysis.
  • The phase's stability and low bleed offer advantages for gradient elution and method development.