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Targeted surface marker screening on neuronal structures in the human choroid.

H Wolfmeier1, S Heindl1, C Platzl1

  • 1Center for Anatomy and Cell Biology, Institute of Anatomy and Cell Biology - Salzburg, Paracelsus Medical University, Salzburg, Austria.

Experimental Eye Research
|December 31, 2022
PubMed
Summary
This summary is machine-generated.

This study identifies specific cluster-of-differentiation (CD) antigens on intrinsic choroidal neurons (ICN), revealing novel molecular markers for choroidal innervation and ocular health research.

Keywords:
CD24/ Heat-stable antigenCD59/ Protectin/ MIRLCluster-of-differentiation/ CDHumanImmunohistochemistryIntrinsic choroidal neurons/ICNSurface marker

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Area of Science:

  • Ophthalmology
  • Neuroscience
  • Immunology

Background:

  • Choroidal neuronal control is vital for retinal and ocular health, but its mechanisms, particularly local innervation by intrinsic choroidal neurons (ICN), are poorly understood.
  • Neuronal function relies on cell surface molecules, yet the specific molecular expression patterns on choroidal neurons remain largely unknown.

Purpose of the Study:

  • To investigate the presence and distribution of various cluster-of-differentiation (CD) antigens on choroidal neuronal structures, with a specific focus on ICN.
  • To identify novel molecular markers on ICN that could elucidate their function and role in ocular health.

Main Methods:

  • Human choroid tissues were analyzed using immunohistochemistry.
  • A pan-neuronal marker (PGP9.5) was used in conjunction with a panel of CD markers.
  • Fluorescence and confocal laser scanning microscopy were employed for detailed imaging and co-localization analysis.

Main Results:

  • Several CD antigens (CD29, CD34, CD56, CD81, CD90, CD146, CD147, CD151, CD171, CD200, CD271) were found co-localized with PGP9.5+ nerve fibers and ICN perikarya.
  • CD24 and CD59 were absent in ICN perikarya but present on some neural processes, suggesting an extrinsic origin for these fibers.
  • The identified CD markers provide a molecular signature for choroidal neurons.

Conclusions:

  • This study provides the first detailed molecular characterization of CD antigens on choroidal neurons, particularly ICN.
  • The findings reveal specific CD markers present on ICN and differentiate them from extrinsic nerve fibers.
  • This molecular atlas opens new avenues for functional studies on choroidal innervation and its impact on ocular health.