Application of Shear Stress to Endothelial Cells Using a Parallel Plate Flow Chamber

Endothelial cell response to shear stress from the flowing blood plays an important role in diseases such as atherosclerosis. It is therefore essential to study how different flow regimes, including both atheroprotective laminar flow and atheropromoting disturbed flow, impact endothelial cell function. Shear stress effects can be studied in vitro using a variety of techniques, each with their own advantages and disadvantages. In this chapter, we describe how to use a parallel plate flow chamber to study the impact of both laminar and disturbed flow on endothelial cell monolayers.