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Related Experiment Video

Updated: Aug 15, 2025

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

Imaging Cell Adhesive Force at the Single Molecule Level.

Ying Tu¹, Xuefeng Wang²

¹Department of Physics and Astronomy, Iowa State University, Ames, IA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 31, 2022

Summary

This summary is machine-generated.

Integrative tension sensors (ITS) visualize and quantify cell adhesive forces. This DNA-based technology converts force into fluorescence, enabling sensitive imaging of cellular mechanics for biological development research.

Keywords:

Integrin Mechanobiology Single molecule imaging Tension sensor dsDNA

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Last Updated: Aug 15, 2025

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

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Published on: September 27, 2021

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Published on: July 10, 2019

Area of Science:

Cellular mechanobiology
Molecular imaging
Biophysics

Background:

Cell adhesive force is crucial for cellular functions and development.
Visualizing and quantifying cell forces is essential in cell mechanobiology.
Existing methods have limitations in sensitivity and resolution.

Purpose of the Study:

To develop a novel ultrasensitive method for visualizing and quantifying cell adhesive force.
To introduce the integrative tension sensor (ITS) for advanced cell mechanobiology imaging.
To enable high-resolution imaging of cellular forces at different scales.

Main Methods:

Development of a dsDNA-based integrative tension sensor (ITS).
Implementation of ITS in two distinct imaging modes: cumulative and real-time.
Utilizing fluorescence conversion to visualize force signals.

Main Results:

ITS successfully converts invisible force signals into detectable fluorescence.
The cumulative mode allows high signal-to-noise ratio imaging even with basic microscopes.
The real-time mode enables imaging of forces at the single molecular tension level.

Conclusions:

Integrative tension sensor (ITS) offers an ultrasensitive platform for cell adhesive force imaging.
ITS provides versatile imaging capabilities for diverse applications in cell mechanobiology.
This technology advances the study of cellular mechanics and biological development.