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Blood Sample Processing and Banking for Functional and Molecular Analyses.

Paméla Thébault1,2, Jean-François Cailhier1,2,3, Réjean Lapointe4,5,6

  • 1Axe Cancer, Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC, Canada.

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PubMed
Summary
This summary is machine-generated.

Proper blood sample collection and processing are crucial for immune monitoring. Standardized procedures ensure sample quality and biomarker stability for accurate research results.

Keywords:
Blood bankingPeripheral blood mononuclear cells (PBMCs)Plasma

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Area of Science:

  • Immunology
  • Biotechnology
  • Clinical Research

Background:

  • Immune monitoring of blood cells offers insights into patient immune responses during disease or treatment.
  • Assessing immune cell function and protein expression is vital for treatment efficacy and disease progression evaluation.
  • Effective immune monitoring relies on meticulous handling and storage of biological samples to maximize information from limited volumes and reduce research costs.

Purpose of the Study:

  • To outline best practices for collecting and processing blood samples for plasma and peripheral blood mononuclear cell (PBMC) banking.
  • To emphasize the importance of sample handling, transport, and storage conditions for maintaining biomarker stability.
  • To provide guidance on study design elements that facilitate downstream genomic, immunological, and biochemical analyses.

Main Methods:

  • Detailed protocols for blood collection, including considerations for sample types, timing, containers, and additives.
  • Procedures for processing blood to isolate plasma and peripheral blood mononuclear cells (PBMC).
  • Guidelines for the storage of biological samples, including cryopreservation of cells.

Main Results:

  • Identified critical factors influencing blood sample quality and biomarker stability, such as transport time and preservatives.
  • Demonstrated the necessity of standardized operating procedures (SOPs) and quality control (QC) plans.
  • Established a framework for efficient sample processing to support diverse downstream analyses.

Conclusions:

  • Careful consideration of pre-analytical variables is essential for reliable immune monitoring.
  • Standardized collection, processing, and banking of blood samples are fundamental for valid research outcomes.
  • Implementing robust SOPs and QC measures safeguards sample integrity and ensures the validity of immunological and genomic data.