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Updated: Aug 15, 2025

Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
Detection of fluorescence-labeled DNA with in-plane organic optoelectronic devices
Igor Titov1, Nils Rutschke2, Fabio A Kraft1
1Integrated Systems and Photonics, Faculty of Engineering, Kiel University, Germany.
Abstract:
We present a system efficiency analysis of a monolithic integrated organic optoelectronic unit for the detection of fluorescence labeled single-stranded DNA (ssDNA) for veterinary disease testing. The side-by-side integration of an organic light emitting diode (OLED) and an organic photodetector (OPD) with 0.5 mm by 0.5 mm device sizes has the potential to enable compact and low-cost fluorescence point-of-care (POC) devices for decentral multiplex biomedical testing. Here, we used two 6-FAM and BHQ1 labeled complementary ssDNA strands to form the Förster resonance transfer (FRET) upon the hybridization of the DNA. In this work we successfully show ssDNA hybridization sensing with samples diluted in TE buffer and investigate the detection of covalently bound 6-FAM-ssDNA on a glass surface for multiplex biomarker measurements.
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