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Published on: February 8, 2016
Probiotic Debaryomyces hansenii CBS 8339 yeast enhanced immune responses in mice
Miriam Angulo1, Abel Ramos1, Martha Reyes-Becerril1
1Immunology and Vaccinology Group, Centro de Investigaciones Biológicas del Noroeste, S.C., Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, C.P. 23096 La Paz, BCS Mexico.
Abstract:
This study aimed to examine the effect of Debaryomyces hansenii CBS 8339 on innate immune responses in mice. Thirty BALB/c mice were randomly treated with phosphate buffered saline (PBS) (control) and two D. hansenii (Dh) doses: Dh 10ˆ6 CFU (colony forming units) and Dh 10ˆ8 CFU daily for 15 days. Spleen, blood, and gut samples were taken on days 7 and 15. Mouse splenocytes were isolated and challenged with Escherichia coli. Immunological assays and immune-related gene expressions were performed. Serum was obtained from blood for total IgA and IgG antibody titer determination. Gut samples were taken for yeast colonization assessment. Phagocytosis, respiratory burst activity, and nitric oxide production in mice were mainly enhanced (p < 0.05) upon 7 days of D. hansenii intake at a concentration of 10ˆ8 CFU before and after bacterial challenge. Moreover, oral D. hansenii in mice upregulated (p < 0.05) gene expression of pro-inflammatory cytokines (INF-γ, IL-6 and IL-1β) before or after E. coli challenge on day 7 but downregulated (p < 0.05) on day 15. Furthermore, total serum IgG and IgA titers were higher (p < 0.05) in Dh 10ˆ8 CFU at days 7 and 15, and only at day 7, respectively, than that in the other dose and control groups. Finally, D. hansenii was detected in the gut of mice that received the treatments, suggesting that yeast survived gastrointestinal transit. Altogether, a short period (7 days) of D. hansenii CBS 8339 oral delivery improved immune innate response on mice.
Insights
Oral administration of Debalomyces hansenii CBS 8339 at a high dose (10^8 CFU) for 7 days significantly enhanced innate immune responses in mice. This yeast improved phagocytosis, cytokine production, and antibody titers, indicating a potential probiotic effect.
Area of Science:
- Immunology
- Microbiology
- Probiotics
Background:
- Innate immunity plays a crucial role in host defense against pathogens.
- Probiotics, including certain yeasts like Debalomyces hansenii, are being investigated for their immunomodulatory effects.
- Understanding the impact of specific yeast strains on immune responses is essential for developing novel health interventions.
Purpose of the Study:
- To investigate the effects of Debalomyces hansenii CBS 8339 on the innate immune system of BALB/c mice.
- To determine the optimal dosage and duration of D. hansenii administration for immune enhancement.
- To evaluate the survival and colonization of D. hansenii in the mouse gastrointestinal tract.
Main Methods:
- BALB/c mice were orally administered with two doses of D. hansenii (10^6 and 10^8 CFU) or PBS (control) daily for 15 days.
- Immune responses were assessed on days 7 and 15, including splenocyte analysis, cytokine gene expression, and serum antibody titers (IgA, IgG).
- Phagocytosis, respiratory burst activity, and nitric oxide production were measured, along with yeast colonization in gut samples.
Main Results:
- A 7-day administration of D. hansenii at 10^8 CFU significantly enhanced phagocytosis, respiratory burst activity, and nitric oxide production.
- Gene expression of pro-inflammatory cytokines (INF-γ, IL-6, IL-1β) was upregulated on day 7 but downregulated on day 15.
- Higher serum IgG and IgA titers were observed in the 10^8 CFU group at days 7 and 15, and day 7, respectively.
Conclusions:
- Short-term oral administration of Debalomyces hansenii CBS 8339 (7 days) effectively improved innate immune responses in mice.
- The higher dose (10^8 CFU) demonstrated a more pronounced immunomodulatory effect, particularly in enhancing cellular and humoral immunity.
- Debalomyces hansenii survived gastrointestinal transit, suggesting its potential as a probiotic agent for immune support.
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