A visual detection strategy for SARS-CoV-2 based on dual targets-triggering DNA walker.

Area of Science:

• Biotechnology
• Nanotechnology
• Molecular Diagnostics

Background:

• The global threat of SARS-CoV-2 necessitates rapid, portable detection methods.
• Current detection strategies often require specialized facilities and equipment, limiting accessibility.
• Point-of-care diagnostics are crucial for early screening and controlling viral spread.

Purpose of the Study:

• To develop a portable, visual detection strategy for the SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) gene.
• To create a method that simplifies the detection process and reduces analysis time.
• To enhance specificity and sensitivity through a dual targets-triggering mechanism and signal amplification.

Main Methods:

• Utilized a silver-coated glass slide (SCGS) integrated with a DNA walker system.
• Employed a dual targets-triggering mechanism for enhanced specificity.
• Incorporated enzyme-catalyzed amplification and Red-Green-Blue (RGB) data analysis via smartphone for visual detection.

Main Results:

• Achieved detection of the SARS-CoV-2 RdRp gene within 30 minutes.
• Demonstrated detection through both ultraviolet absorbance and visible color changes.
• Verified the method's practicability using real-world samples, showing potential for rapid SARS-CoV-2 detection.

Conclusions:

• The proposed SCGS/DNA walker system offers a simplified, rapid, and portable visual detection strategy for SARS-CoV-2.
• The dual targets-triggering and dual signal amplification enhance detection accuracy.
• This method holds significant application potential for on-site and early screening of SARS-CoV-2 infections.