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Tissue reconstruction of abdominal wall with butyric acid-based nets: preliminary in vitro test using tissue
I Zanolla1, E Tiengo, F Zanotti
1Department of Translational Medicine, University of Ferrara, Ferrara, Italy. barbara.zavan@unife.it.
Objective:
A hernia of the abdominal wall is an opening of the muscles in the abdominal wall, which is frequently treated via the application of a surgical mesh. The purpose of this research is to study how human adipose-derived stem cells (hADSCs) interact with Phasix™ Mesh, a commercially available mesh for hernia repair. Studying how cells derived from the abdominal region behave with Phasix™ Mesh is crucial to improve the state of the art of current surgery and achieve effective tissue restoration.
Materials And Methods:
hADSCs were seeded onto Phasix™ Mesh, a fully resorbable surgical mesh of poly (4-hydroxybutyric acid) (P4HB). Cell viability was assessed through MTT assay, and cell growth and adhesion were evaluated via multiple imaging techniques and gene imaging profiling.
Results:
Results confirm that the nets support cells proliferation, extracellular matrix production and increasing of angiogenetic factor.
Conclusions:
Butyric acid-based nets are promising scaffolds for abdominal wall reconstruction.
