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Related Experiment Video

Updated: Aug 15, 2025

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Targeted Mutagenesis in Mice Using a Base Editor.

Tae Yeong Jeong¹, Soo-Yeon Lim², Je Kyung Seong^2,3,4,5

¹Department of Biomedical Sciences and Department of Physiology, Korea University College of Medicine, Seoul, Republic of Korea.

Methods in Molecular Biology (Clifton, N.J.)

|January 2, 2023

Summary

This summary is machine-generated.

This study presents a novel base editing system for generating mouse models with precise point mutations. This CRISPR-based technology enables specific nucleotide substitutions, advancing genome editing capabilities.

Keywords:

Adenine base editor CRISPR system Cytosine base editor Mouse model generation Nucleotide substitution Targeted mutagenesis

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Last Updated: Aug 15, 2025

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Published on: April 2, 2014

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

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Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

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Published on: March 16, 2022

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Base editors are CRISPR-based tools utilizing nickase Cas9 (nCas9) and deaminase for specific nucleotide substitutions.
Cytosine and adenine base editors are effective for C-to-T and A-to-G conversions.
An enhanced base editor, C-to-G base editor (CGBE), expands capabilities to C-to-G conversions.

Purpose of the Study:

To introduce a novel method for generating mouse models with point mutations.
To utilize an advanced base editing system for precise genome editing.
To demonstrate the application of base editing in creating specific point mutations in mice.

Main Methods:

Development of a base editing system for generating mouse models.
Application of CRISPR-based enzymatic tools for nucleotide substitutions.
Utilizing enhanced base editors for specific point mutation induction.

Main Results:

Successful generation of mouse models with point mutations using the base editing system.
Demonstration of precise nucleotide substitutions, including C-to-G conversions.
Establishment of a method for efficient point mutation introduction in mice.

Conclusions:

The developed base editing system is effective for generating mouse models with point mutations.
This method expands the utility of CRISPR-based genome editing tools.
The study provides a valuable approach for creating precise genetic modifications in mice.