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Updated: Aug 15, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
Published on: October 18, 2022
Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor
Seu-Na Lee1, Hong-Su Jang1, Jae-Sung Woo2
1Department of Life Sciences, Korea University, Seoul, Republic of Korea.
Abstract:
CRISPR-cas9-guided adenine base editors (ABEs) site-specifically convert the A-T base pair to G-C base pair in genomic DNA. The intracellular delivery of ABE proteins preassembled with guide RNAs (gRNAs) has shown greatly reduced off-target effects compared with that of plasmids or viral vectors containing ABE and gRNA-encoding sequences. For efficient gene editing by the ribonucleoprotein delivery method, the ABE-gRNA complexes need to be prepared in high purity and quantity. Here we describe the expression and purification procedure of ABEmax, one of high-efficiency ABE versions.
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