Spirometra mansoni sparganosis identified by metagenomic next-generation sequencing: a case report
Diefei Hu1, Wenxiang Jin2, Huarong Ding3
1Department of Infectious Diseases, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
Abstract:
A 30-year-old male patient had a cyst on the left hip and progressive enlargement for more than 2 months. Combined blood tests, magnetic resonance imaging, and pathology findings, cysticercosis infection was suspected. However, the treatment for cysticercosis was ineffective. We conducted a metagenomic next-generation sequencing (mNGS) analysis on the formalin-fixed, paraffin-embedded specimen of the patient's surgically excised tissue, and the results suggested Spirometra mansoni, mNGS was further confirmed by polymerase chain reaction and phylogenetic analysis of cytochrome c oxidase subunit 1 (cox1) gene. Based on these results, we found that mNGS provided a better method of diagnosing parasitic infections.
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