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Updated: Aug 15, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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A novel fully-automated method to measure steroids in serum by liquid chromatography-tandem mass spectrometry

François Fraissinet1, Tony Pereira1, Alizée Violin2

  • 1Department of General Biochemistry, Rouen University Hospital, 76000 Rouen, France.

Journal of Mass Spectrometry and Advances in the Clinical Lab
|January 3, 2023
PubMed
Abstract

Keywords:
11DF, 11-deoxycortisol17OHP, 17-hydroxyprogesterone2D-UHPLC-MS/MS, Two dimensional ultra-high performance liquid chromatography-tandem mass spectrometryAutomationD4, delta4-androstenedioneDHEA, dehydroepiandrosteroneEMA, European Medicine AgencyGC–MS/MS, Gas chromatography tandem mass spectrometryLC-MS/MS, Liquid chromatography tandem mass spectrometryLLE, Liquid-liquid extractionLLOQ, Lower limit of quantificationLiquid chromatography tandem mass spectrometryMRM, Multiple reaction monitoringPTFE, PolytetrafluoroethyleneQC, Quality controlRIA, RadioimmunoassayRadioimmunoassaySLE, Supported liquid extractionSPE, Solid phase extractionSRM, Standard reference materialSteroidsT, TestosteroneTestosteroneUHPLC, Ultra-high performance liquid chromatography

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