Related Experiment Video
Updated: Aug 15, 2025

Author Spotlight: Innovating Thiol Quantification and Biomarker Detection for Oxidative Stress Research
Published on: June 28, 2024
Reversible dynamic optical sensing based on coumarin modified β-cyclodextrin for glutathione in living cells
Zhixue Liu1, Mengdi Tian2, Heng Zhang2
1College of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China. yuliu@nankai.edu.cn.
Abstract:
Coumarin acting as an optical probe was modified on ethylenediamine β-cyclodextrin, which not only enhanced its molecular binding affinity to glutathione (GSH) by a reversible Michael addition, showing 113 times more affinity than that of coumarin itself, but also achieved dynamic real-time sensing of glutathione in living HeLa cells.
More Related Videos
07:47Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Published on: October 20, 2021
14:43Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
Published on: September 23, 2013