Related Experiment Video
Updated: Aug 15, 2025
Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
Published on: November 4, 2019
Generation of expandable cardiovascular progenitor cells from mouse and human fibroblasts via direct chemical
Jia Wang1, Qiyuan Wang2, Nan Cao2
1School of Health and Life Sciences, University of Health and Rehabilitation Sciences, Qingdao City, Shandong Province 266071, China.
Abstract:
Here, we present a protocol to reprogram mouse and human fibroblasts into expandable cardiovascular progenitor cells (CPCs) via a defined small-molecule treatment. We describe steps to prepare fibroblasts and generate the chemically induced CPCs (ciCPCs), followed by expansion and differentiation of the ciCPCs. These cells can self-renew in the long term, faithfully retaining the CPC phenotype and cardiovascular differentiation capacity. This protocol provides an autologous and expandable cardiovascular cell source, which may find uses in cardiovascular disease modelling, drug discovery, and cardiac cell therapy. For complete details on the use and execution of this protocol, please refer to Wang et al. (2022).1.
