Related Experiment Video
Updated: Aug 15, 2025

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
Published on: August 6, 2018
High-throughput automated platform for thin layer chromatography analysis
Hao Xu1, Dongxiao Zhang2, Fanyang Mo3
1School of Materials Science and Engineering, Peking University, Beijing 100871, China; BIC-ESAT, ERE, and SKLTCS, College of Engineering, Peking University, Beijing 100871, China.
Abstract:
In this protocol, we detail steps for constructing a high-throughput automated platform for thin layer chromatography (TLC) analysis. We describe robotics and computer vision techniques that can handle 32 compounds under three different elution solvents in about 50 min. The established automated platform can obtain statistically standardized retardation factor (Rf) values and enhance reproducibility while reducing labor and time costs. For complete details on the use and execution of this protocol, please refer to Xu et al. (2022).1.
More Related Videos
12:04Thin-layer Chromatographic TLC Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
Published on: March 27, 2014
07:34Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
Related Concept Videos
Thin-Layer Chromatography (TLC): Overview
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process: