Analysis of Methamphetamine in Urine by HPLC with Solid-Phase Dispersive Extraction and Solid-Phase Fluorescence...

Area of Science:

• Analytical Chemistry
• Forensic Science

Background:

• Accurate methamphetamine detection in urine is crucial for clinical and forensic applications.
• Conventional methods can be time-consuming and may lack stability in reaction products.

Purpose of the Study:

• To develop a simple, rapid, and reliable method for methamphetamine analysis in urine.
• To improve the stability of reaction products compared to liquid-phase methods.

Main Methods:

• Developed a fluorescence detection-liquid chromatography (HPLC-FL) method.
• Employed solid-phase dispersive extraction (SPDE) for sample cleanup.
• Utilized solid-phase fluorescence derivatization with 9-fluorenylmethyl chloroformate (FMOC).

Main Results:

• Achieved high recoveries of methamphetamine (83.6% at 100 ng/mL, 80.7% at 1000 ng/mL).
• Obtained clean chromatograms with no interfering peaks, indicating method validity.
• Demonstrated enhanced stability of the FMOC-MA reaction product compared to liquid-phase methods.

Conclusions:

• The developed HPLC-FL method with SPDE and solid-phase derivatization offers a simple and rapid approach for urine methamphetamine analysis.
• The method provides reliable quantification and improved product stability.
• This technique is a valuable advancement for forensic and clinical toxicology.