In Situ Tracking of Crystal-Surface-Dependent Cu₂O Nanoparticle Dissolution in an Aqueous Environment

Metal-oxide-based nanoparticles (MONPs) such as Cu₂O NPs have attracted growing attention, but the potential discharges of MONPs have raised considerable concern of their environmental fate including their dissolution behavior. The impacts of morphology on MONP dissolution are largely uncertain due to the lack of in situ tracking techniques. In this study, we combined a series of in situ technologies including liquid-cell transmission electron microscopy and fluorescence probes to reveal the in situ dissolution process of Cu₂O NPs in freshwater. Our results suggest that cubic Cu₂O NPs exhibit a higher dissolution quantity compared with spherical NPs of the same surface area. The difference was mainly related to the crystal surface, while other factors such as particle size or aggregation status showed minor effects. Importantly, we demonstrated the simultaneous growth of new small NPs and the dissolution of pristine Cu₂O NPs during the dissolution of Cu₂O NPs. Cubic Cu₂O NPs became much less soluble under O₂-limited conditions, suggesting that O₂ concentration largely affected the dependence of dissolution on the NP morphology. Our findings highlight the potential application of in situ techniques to track the environmental fates of MONPs, which would provide important information for assessing the ecological risks of engineered NPs.