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Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
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Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
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Regulation of Human Platelet Adhesion with Ascorbic Acid.

M S Makarov1, M V Storozheva2

  • 1N. V. Sklifosovsky Research Institute of Emergency Medicine, Moscow Health Department, Moscow, Russia. mcsimmc@yandex.ru.

Bulletin of Experimental Biology and Medicine
|January 4, 2023
PubMed
Summary

Ascorbic acid at low concentrations (0.5-1 mM) preserves platelet granules and suppresses degranulation. However, higher concentrations (2-5 mM) stimulate spontaneous platelet activation and degranulation.

Keywords:
ascorbic aciddegranulationlamellae growthplatelets

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Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor
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Area of Science:

  • Biochemistry
  • Hematology
  • Cell Biology

Background:

  • Platelets are crucial for hemostasis and thrombosis.
  • Ascorbic acid (vitamin C) is a potent antioxidant with potential roles in platelet function.
  • Understanding ascorbic acid's effects on platelet morphofunctional characteristics is important for clinical applications.

Purpose of the Study:

  • To investigate the dose-dependent effects of ascorbic acid on human platelet structure and function.
  • To evaluate the impact of ascorbic acid on platelet lamella formation, granule preservation, and adhesion.
  • To determine optimal ascorbic acid concentrations for stabilizing platelet granules.

Main Methods:

  • Human platelets were incubated with varying concentrations of ascorbic acid (0.1-5 mM).
  • Platelet morphofunctional characteristics, including lamella formation and granule preservation, were assessed.
  • Platelet adhesion activity was evaluated.
  • Changes in platelet structure and degranulation were observed under microscopy.

Main Results:

  • Low ascorbic acid concentrations (0.1-1 mM) did not alter platelet structure or adhesion but dose-dependently suppressed lamella growth and degranulation in adherent platelets.
  • Maximum granule preservation (55% at 1 hour) was observed at 0.5 mM ascorbic acid.
  • High ascorbic acid concentrations (2-5 mM) induced spontaneous platelet activation and degranulation.

Conclusions:

  • Ascorbic acid exhibits dual effects on platelet activity, capable of both suppression and stimulation.
  • Concentrations of 0.5-1 mM ascorbic acid can effectively stabilize granules in adherent platelets.
  • These findings suggest potential therapeutic applications for ascorbic acid in managing platelet-related disorders.