Growth-Stimulating Effect of Platelet Lysates Obtained with Low-Pulse Laser Radiation in Human Fibroblast Culture

The growth-stimulating effect of platelet lysates obtained after exposure of platelet suspension to low-pulse laser radiation (LPLR) with λ = 470 nm (blue laser) and λ = 635 nm (red laser) was studied in a culture of human M-22 fibroblasts. LPLR-irradiated platelet suspension in plasma and plasma-free medium reduced the content of platelets with granules by 5-7% at λ = 470 nm and 25-27% at λ = 635 nm (p < 0.05). In presence of platelet lysates, the number of fibroblasts significantly increased by 1.3-1.4 times after 3 days of cultivation. In experiments with platelets in plasma-free medium, the samples previously irradiated at λ = 470 nm produced a lower growth-stimulating effect, than samples irradiated at λ = 635 nm and non-irradiated samples; for platelets irradiated in autologous plasma, no prominent differences were observed. Lysates of LPLR-irradiated platelets did not impair structural integrity of human fibroblasts in culture.