Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Benefits of a sustained community-based psychiatric intervention on viral exposure among people who inject drugs: The ANRS Drive-Mind 2 cohort study in Hai Phong, Vietnam.

PLOS mental health·2026

Same author

Altair-dvOPM: an open-access platform for large-field three-dimensional tissue imaging.

bioRxiv : the preprint server for biology·2026

Same author

A High-Throughput Platform for Rapid Adaptation of DNA Aptamers to SARS-CoV-2 Evolution.

bioRxiv : the preprint server for biology·2026

Same author

p-STAT3 expression associates with prognosis and inflammatory indexes in gastric cancer patients.

Scientific reports·2026

Same author

DICER cleavage fidelity is governed by 5'-end binding pockets.

Nature·2026

Same author

Quantifying the speed-accuracy trade-off of large language models on oral and maxillofacial surgery multiple-choice questions.

Scientific reports·2025

Search research articles

Related Experiment Video

Updated: Aug 15, 2025

Hand Dissection of Caenorhabditis elegans Intestines

Hand Dissection of Caenorhabditis elegans Intestines

Published on: September 13, 2022

Dissection of the Caenorhabditis elegans Microprocessor.

Thuy Linh Nguyen¹, Trung Duc Nguyen¹, Minh Khoa Ngo¹

¹Division of Life Science, The Hong Kong University of Science & Technology, Hong Kong, China.

Nucleic Acids Research

|January 4, 2023

Summary

This summary is machine-generated.

The microRNA processing complex (MP) mechanism in Caenorhabditis elegans differs from humans. Researchers uncovered how cDrosha and Pasha proteins measure pri-miRNA stems to determine cleavage sites, revealing distinct miRNA biogenesis pathways.

More Related Videos

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

Published on: April 11, 2022

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Published on: April 19, 2018

Related Experiment Videos

Last Updated: Aug 15, 2025

Hand Dissection of Caenorhabditis elegans Intestines

Hand Dissection of Caenorhabditis elegans Intestines

Published on: September 13, 2022

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

Published on: April 11, 2022

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Published on: April 19, 2018

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

MicroRNAs (miRNAs) are crucial regulators of gene expression at the post-transcriptional level.
The Microprocessor complex (MP), comprising DROSHA and DGCR8/Pasha, initiates miRNA biogenesis by processing pri-miRNAs.
Understanding MP mechanisms is vital for comprehending miRNA roles in cellular processes and diseases.

Purpose of the Study:

To elucidate the molecular mechanism of the Caenorhabditis elegans Microprocessor complex (cMP).
To compare the cMP mechanism with the well-characterized human MP (hMP).
To identify the distinct features of cMP in pri-miRNA processing.

Main Methods:

Biochemical assays to analyze the interaction of cDrosha and Pasha with pri-miRNAs.
Determination of the specific binding sites and lengths measured by cDrosha and Pasha on pri-miRNA stems.
Comparative analysis of cMP and hMP substrate recognition and cleavage.

Main Results:

The cMP complex exhibits a distinct mechanism compared to hMP.
cDrosha measures approximately 16 base pairs and Pasha measures approximately 25 base pairs along the pri-miRNA stem.
These measurements dictate the precise cleavage site selection by cMP.

Conclusions:

A novel molecular mechanism for cMP-mediated pri-miRNA processing has been revealed.
Significant mechanistic differences exist between cMP and hMP.
This study provides a foundation for understanding species-specific regulation of miRNA biogenesis.