Related Experiment Video
Updated: Aug 15, 2025
Laser Doppler Perfusion Imaging in the Mouse Hindlimb
Published on: April 18, 2021
Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill
Stephen P Ashcroft1, Sara L Jepsen2, Amy M Ehrlich1
1Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Abstract:
The tissue-specific release and uptake of metabolites in response to exercise is incompletely understood. Here, we detail a protocol to assess arteriovenous differences across the liver and hindlimb muscles in response to treadmill exercise in mice. We describe steps for the treadmill running of mice and the region-specific sampling of blood from the liver and hindlimb. This procedure is particularly relevant for the study of tissue-specific metabolism in response to exercise. For complete details on the use and execution of this protocol, please refer to Sato et al. (2022).1.
