A ratiometric fluorescent nanoprobe based on CdSe quantum dots for the detection of Ag⁺ in environmental samples and living cells

With the widespread application of Ag⁺ in modern life and industry, the potential hazardous effects of Ag⁺ to environment and humans have attracted great concerns. Thus, effective and rapid strategies for Ag⁺ detection are highly desirable. In this paper, a novel ratiometric fluorescence sensor using CdSe quantum dots (QDs) has been constructed for sensitive and selective detection of Ag⁺, which is based on the formation of Ag₂Se QDs. CdSe QDs were initially prepared and showed single wavelength emission at 510 nm. When Ag⁺ exists, a rising peak appeared at 650 nm and the emission at 510 nm declined, exhibiting distinct ratiometric fluorescence emission (I₆₅₀/I₅₁₀) characteristic with a linear response over the Ag⁺ concentration range of 0.01-4 μM. Significantly, the fluorescence changed from green to red. The detection limit of the constructed sensor is 1.4 nM. Furthermore, the sensing assay can be successfully applied to detect Ag⁺ in real water samples and living cells.