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Related Concept Videos

Smallpox01:24

Smallpox

Smallpox is a severe contagious disease caused by the Variola major virus, a double-stranded DNA member of the Poxviridae family.Variola major transmission occurs primarily via inhalation of virus-laden droplets or direct contact with infectious scabs. The incubation period averages approximately seven days, although it may range from 7 to 17 days depending on the inoculum and host factors.Clinically, the prodromal phase is marked by an abrupt onset of high fever, malaise, headache, and myalgia.
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Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
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Inhibitors Of Virion Release

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Antiviral Nucleoside Inhibitors01:22

Antiviral Nucleoside Inhibitors

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Inhibitors of Virion Maturation and Assembly01:19

Inhibitors of Virion Maturation and Assembly

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Silver nanoparticles inhibit goatpox virus replication.

Mohamed J Saadh1

  • 1Faculty of Pharmacy, Middle East University, Amman, Jordan. msaadeh@meu.edu.jo.

Archives of Virology
|January 5, 2023
PubMed
Summary

Silver nanoparticles (AgNPs) show promise in combating goatpox virus (GTPV), a significant threat to livestock. This study demonstrates AgNPs effectively inhibit GTPV replication by preventing viral entry into host cells, offering a potential new therapeutic strategy.

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Area of Science:

  • Veterinary Virology
  • Nanomedicine
  • Antiviral Research

Background:

  • Goatpox virus (GTPV) causes severe disease with high mortality in goats, and no effective treatments are currently available.
  • The development of novel antiviral strategies is crucial for managing GTPV outbreaks and protecting livestock populations.

Purpose of the Study:

  • To evaluate the antiviral activity of biologically synthesized silver nanoparticles (AgNPs) against goatpox virus (GTPV) in vitro.
  • To investigate the mechanism of AgNPs' antiviral action against GTPV, focusing on viral entry and replication.

Main Methods:

  • Biosynthesis of AgNPs using Piper betle leaf extract and silver nitrate.
  • Characterization of AgNPs using UV-vis spectroscopy, XRD, FTIR, and TEM.
  • Antiviral assays involving titration of GTPV (TCID50/mL) and quantification of viral genome copy number following AgNP treatment.

Main Results:

  • AgNPs demonstrated significant inhibition of GTPV replication in vitro.
  • AgNPs primarily acted by preventing the entry of GTPV into host cells.
  • AgNPs also led to a significant reduction in the viral genome copy number.

Conclusions:

  • Biologically synthesized AgNPs exhibit potent antiviral activity against GTPV.
  • AgNPs represent a potential therapeutic agent for goatpox, acting through mechanisms that inhibit viral entry and replication.