High-performance liquid chromatography using diode array detector and fluorescence detector for hydrogen peroxide...

Area of Science:

• Food chemistry
• Analytical chemistry
• Chromatography

Background:

• Hydrogen peroxide (H2O2) is utilized as a food additive for bleaching and sterilization due to its potent oxidizing properties.
• Ensuring food safety necessitates accurate methods for detecting residual H2O2 in various food products.

Purpose of the Study:

• To develop and validate analytical methods for the precise detection of trace amounts of residual hydrogen peroxide in diverse food matrices.
• To compare the performance of high-performance liquid chromatography with diode array detection (HPLC-DAD) and fluorescence detection (HPLC-FLD) for H2O2 analysis.

Main Methods:

• Detection of H2O2 using HPLC-DAD via the formation of a vanadium(V)-peroxo complex.
• Indirect analysis of H2O2 with HPLC-FLD by detecting 7-hydroxycoumarin produced via a Fenton reaction.
• Verification of results using liquid chromatography with tandem mass spectrometry (LC-MS/MS).

Main Results:

• Both HPLC-DAD and HPLC-FLD methods demonstrated excellent linearity (R² > 0.997).
• HPLC-FLD exhibited superior sensitivity with limits of detection and quantification of 0.001 mg/L and 0.003 mg/L, respectively.
• The developed methods were successfully applied to analyze H2O2 in real food samples.

Conclusions:

• Sensitive and reliable HPLC-based methods were established for quantifying residual hydrogen peroxide in foods.
• HPLC-FLD offers enhanced sensitivity for trace H2O2 detection compared to HPLC-DAD.
• These methods provide valuable tools for food safety monitoring and quality control.