Target-activated cascade transcription amplification lights up RNA aptamers for label-free detection of metalloproteinase-2 activity
Related Experiment Video
Updated: Aug 15, 2025

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
Ning-Ning Zhao1, Wen-Jing Liu2, Xiaorui Tian1
1College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China. cyzhang@sdnu.edu.cn.
Abstract:
We demonstrate that target-activated cascade transcription amplification lights up RNA aptamers for label-free detection of metalloproteinase-2 (MMP-2) activity with zero background. This assay exhibits good specificity and high sensitivity with a limit of detection (LOD) of 0.6 fM. Moreover, it can analyze enzyme kinetic parameters, screen inhibitors, and accurately quantify MMP-2 in cancer cells and clinical serums.

