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Yeast Mannan-Rich Fraction Modulates Endogenous Reactive Oxygen Species Generation and Antibiotic Sensitivity in

Helen Smith1, Sharon Grant1, Paula Meleady2

  • 1Alltech, Summerhill Road, Dunboyne, A86X006 Meath, Ireland.

International Journal of Molecular Sciences
|January 8, 2023
PubMed

Insights

Mannan-rich fraction (MRF) from Saccharomyces cerevisiae boosts reactive oxygen species (ROS) in resistant E. coli, increasing antibiotic susceptibility. MRF also alters bacterial metabolism and protein expression, enhancing treatment efficacy.

Area of Science:

  • Microbiology
  • Biochemistry
  • Animal Science

Background:

  • Mannan-rich fraction (MRF) from Saccharomyces cerevisiae is known for promoting animal gut health.
  • Antibiotic resistance in bacteria like E. coli poses a significant global health challenge.
  • Understanding novel strategies to combat resistant bacteria is crucial.

Purpose of the Study:

  • To investigate the effect of MRF on reactive oxygen species (ROS) production in antibiotic-resistant E. coli.
  • To determine how MRF influences bacterial metabolism and proteomic changes.
  • To evaluate MRF's potential to enhance antibiotic susceptibility in resistant E. coli.

Main Methods:

  • Proteomics-based approach to analyze protein expression changes.
  • Measurement of ROS production in E. coli cultures.
  • Assessment of E. coli growth and antibiotic susceptibility in the presence of MRF.

Main Results:

  • MRF combined with antibiotics significantly increased ROS production in resistant E. coli by 59.29% (p ≤ 0.05).
  • MRF supplementation impacted translational control of central metabolic pathways, including glycolysis and the TCA cycle.
  • MRF potentiated bacterial susceptibility to antibiotics through modulation of key metabolic pathways.

Conclusions:

  • MRF enhances the efficacy of antibiotics against resistant E. coli by increasing ROS production and altering bacterial metabolism.
  • MRF shows potential as a supportive agent in combating antibiotic resistance.
  • Further research into MRF's mechanisms could lead to new therapeutic strategies.
Keywords:
antibiotic susceptibilityantimicrobial resistancebacterial metabolismreactive oxygen speciesyeast mannan fraction

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