Fabrication of Bio-Based Film Comprising Metal Oxide Nanoparticles Loaded Chitosan for Wound Dressing Applications

In the current work, chitosan (CS)-metal oxide nanohybrid (MONH) composites are prepared via combining CS with MONH made of vanadium oxide (V₂O₅), ytterbium trioxide (Yb₂O₃), and graphene oxide (GO) to generate promising wound dressing materials using the film-casting method. The developed nanohybrid@CS was examined using techniques such as Fourier-transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), scanning electron microscopy (SEM)/energy-dispersive X-ray spectroscopy (EDX), and thermogravimetric analysis (TGA). For Yb₂O₃@CS, the surface morphology was shown to be a rough and porous surface with pores that ranged in size from 3.0 to 5.0 µm. For CS with Yb₂O₃, Yb₂O₃/V₂O₅@CS, and Yb₂O₃/V₂O₅/GO@CS, the contact angles were 72.5°, 68.2°, and 46.5°, respectively. When the nanohybrid@CS was in its hydrophilic phase, which is good for absorbing moisture and drugs, there was a notable decrease in angles that tended to rise. Additionally, the inclusion of MONH allowed the cell viability to be confirmed with an IC₅₀ of 1997.2 g/mL and the cell growth to reach 111.3% at a concentration of 7.9 g/mL.