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A Smartphone-Based Disposable Hemoglobin Sensor Based on Colorimetric Analysis.

Zhuolun Meng1, Muhammad Tayyab1, Zhongtian Lin1

  • 1Department of Electrical and Computer Engineering, Rutgers University, Piscataway, NJ 08854, USA.

Sensors (Basel, Switzerland)
|January 8, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a novel disposable biosensor for accurate hemoglobin quantification in whole blood using smartphone colorimetric analysis. The device offers a portable and cost-effective solution for diagnosing various blood-related diseases.

Keywords:
biosensorhemoglobinlab-on-a-chipmedical tapemicropumprapid testsmartphone colorimetric measurement

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Point-of-Care Diagnostics

Background:

  • Hemoglobin levels are critical biomarkers for diagnosing anemia, sickle cell disease, and thalassemia.
  • Accurate and accessible hemoglobin quantification is essential for disease management.
  • Existing methods can be complex and require specialized laboratory equipment.

Purpose of the Study:

  • To develop a disposable, smartphone-based biosensor for accurate hemoglobin quantification.
  • To enable point-of-care hemoglobin measurement using colorimetric analysis.
  • To provide a cost-effective and user-friendly diagnostic tool.

Main Methods:

  • A microfluidic chip fabricated with medical-grade tapes and filter paper on a glass slide.
  • A custom PolyDimethylSiloaxane (PDMS) micropump for controlled capillary flow.
  • Smartphone imaging with a 3D-printed attachment featuring an LED light source for consistent illumination.
  • Colorimetric analysis of RGB values from blood images to quantify hemoglobin concentration.

Main Results:

  • Demonstrated successful hemoglobin quantification in bovine hemoglobin powder, frozen beef blood, and human blood.
  • Developed a logarithmic model correlating Red channel RGB values with hemoglobin concentration.
  • The device achieved accurate hemoglobin level measurements through colorimetric analysis.

Conclusions:

  • The developed disposable biosensor offers a promising approach for accurate, smartphone-based hemoglobin quantification.
  • This technology has the potential for widespread application in point-of-care settings for disease diagnosis and monitoring.
  • The device provides a portable, cost-effective, and accessible method for assessing hemoglobin levels.