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Updated: Aug 15, 2025

Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis

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Published on: October 19, 2021

Comparative Embryo Development Outcomes following Extending Embryo Culture to Day 6: A Retrospective Cohort Study.

Clara Q Wu^1,2,3, Molly Campbell⁴, Doron Shmorgun^1,2

¹Department of Obstetrics and Gynecology, University of Ottawa, Ottawa, Canada.

International Journal of Fertility & Sterility

|January 8, 2023

Summary

Extending embryo culture to day 6 for vitrification did not increase the proportion of eligible embryos per IVF cycle. However, the mean number of cryopreserved embryos increased significantly.

Keywords:

Blastocyst Embryo Development Vitrification delayed blastulation

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Last Updated: Aug 15, 2025

Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis

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Mouse Embryonic Development in a Serum-free Whole Embryo Culture System

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Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

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Area of Science:

Reproductive medicine
Embryology
Assisted reproductive technology

Background:

Culturing slow-growing embryos to day 6 may improve vitrification success.
This study investigates if extending culture to day 6 increases the proportion of embryos eligible for vitrification.

Purpose of the Study:

To evaluate the impact of a cryopreservation policy change on embryo vitrification eligibility.
To identify factors predicting day 6 embryo development and vitrification.

Main Methods:

Retrospective cohort study of 679 IVF cycles (August 2019 - December 2020).
Compared outcomes before and after a policy change allowing day 6 blastocyst vitrification.
Analyzed embryo developmental outcomes and predictive factors.

Main Results:

No significant difference in the proportion of blastocysts eligible for cryopreservation per cycle (46.9% vs. 44.4%).
Significantly higher mean number of cryopreserved embryos in the group with the updated policy (2.2 vs. 1.7).
Younger egg provider age, early day 5 blastocyst, and surgically retrieved sperm predicted day 6 progression.

Conclusions:

A policy change including day 6 blastocysts yielded comparable embryo eligibility proportions per IVF cycle.
The updated policy led to a higher number of cryopreserved embryos.
Identified key factors influencing day 6 embryo development.