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Related Concept Videos

Nephrons01:10

Nephrons

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The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...
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Renal Drug Excretion: Tubular Secretion01:28

Renal Drug Excretion: Tubular Secretion

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Active tubular secretion is a robust, energy-demanding process that utilizes carrier systems to transport drugs into renal tubules. The active renal secretion systems include the organic anion transporter (OAT) for weak acids and the organic cation transporter (OCT) for weak bases. Structurally similar drugs can compete for the same transporter, potentially leading to drug accumulation and toxicity. However, this principle can be exploited therapeutically. One example is probenecid (Probalan),...
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Physiology of the Genitourinary System III: Urine Concentration and Dilution01:20

Physiology of the Genitourinary System III: Urine Concentration and Dilution

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The kidneys concentrate or dilute urine to maintain water and electrolyte balance. Nephrons, particularly the loop of Henle, play a crucial role in this process through the countercurrent multiplication system. This system establishes a high osmolarity in the renal medulla, which is essential for water reabsorption. In the loop of Henle’s descending limb, water is reabsorbed into the surrounding medulla due to its permeability to water. In contrast, the ascending limb actively transports...
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Physiology of the Genitourinary System I: Renal Blood Flow and Glomerular Filtration01:29

Physiology of the Genitourinary System I: Renal Blood Flow and Glomerular Filtration

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The kidneys are vital organs responsible for regulating blood filtration, waste excretion, and fluid balance, all of which are crucial for maintaining homeostasis. Renal physiology examines renal blood flow, glomerular filtration, and urine formation, ensuring the body’s internal environment remains stable.Renal Blood FlowThe kidneys receive about 20-25% of the cardiac output, typically around 1200 mL of blood per minute in an average adult. Blood flows into the kidneys through the renal...
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Renal Tubule and Collecting Duct01:24

Renal Tubule and Collecting Duct

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The renal tubule is divided into three parts: the proximal convoluted tubule (PCT), the Loop of Henle (LOH), and the distal convoluted tubule (DCT).
Proximal Convoluted Tubule (PCT):
The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
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Formation of Dilute Urine01:20

Formation of Dilute Urine

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The formation of dilute urine is a critical renal adaptation that maintains fluid balance, particularly during periods of high fluid intake. This process primarily involves the juxtamedullary nephrons. By adjusting the permeability of water and ions in response to physiological conditions, the kidneys can either conserve or excrete water, resulting in concentrated or dilute urine.
Filtrate Osmolarity in the PCT
Initially, as the filtrate passes through the proximal convoluted tubule (PCT), its...
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Related Experiment Video

Updated: Aug 14, 2025

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
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Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells

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Selected renal cells harbor nephrogenic potential.

Prakash Narayan1, Andrew T Bruce1, Elias A Rivera1

  • 1Department of Bioprocess Research and Development, ProKidney LLC, Winston-Salem, NC, United States.

Frontiers in Medicine
|January 9, 2023
PubMed
Summary
This summary is machine-generated.

Selected renal cells (SRCs) show potential for chronic kidney disease treatment by promoting kidney development and repair. These cells form tubules and aid in glomerular development, improving kidney function in preclinical models.

Keywords:
celldiseaseglomerulikidneynephrogenicorganoidstherapytubules

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Area of Science:

  • Nephrology
  • Regenerative Medicine
  • Cell Biology

Background:

  • Selected renal cells (SRCs) are a promising autologous cell therapy for chronic kidney disease (CKD).
  • The precise mechanisms behind SRCs' renal reparative effects require further investigation.

Purpose of the Study:

  • To elucidate the molecular mechanisms and functional potential of SRCs in renal development and repair.
  • To investigate the therapeutic efficacy of SRCs in a preclinical model of kidney injury.

Main Methods:

  • Integrated knowledgebase data with empirical findings to analyze SRC gene expression.
  • Cultured SRCs to form organoids and assessed their self-assembly properties.
  • Implanted SRCs into subtotally nephrectomized rats to evaluate in vivo effects on renal structure and function.

Main Results:

  • SRC genes form interactomes involved in renal development, including epithelial differentiation and vascularization.
  • In vitro, SRCs self-assembled into tubular structures within scaffolds.
  • In vivo, SRCs promoted comma- and S-shaped body formation, glomerular development, and improved renal filtration and microarchitecture in rats.

Conclusions:

  • SRCs possess nephrogenic potential, contributing to renal development and repair.
  • These findings provide mechanistic insights into SRC-based therapies for CKD.
  • SRCs demonstrate therapeutic promise for restoring kidney structure and function.