Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Paradigm Shift of Microbiota-gut-brain Axis During Aging: Potential Role of Probiotics to Improve Cognitive Decline.

Probiotics and antimicrobial proteins·2026
Same author

Genomic diversity, antibiotic resistance, and maturation‑dependent adhesion of F18 enterotoxigenic Escherichia coli strains in porcine intestinal cells.

Gut pathogens·2026
Same author

Application of the solvent effect on bioluminescent reporter bacteria as a real-time membrane toxicity assay.

Access microbiology·2026
Same author

Diverse reference genomes detect variants in the US winter wheat.

The plant genome·2025
Same author

Association Mapping for Biomass and Kernel Traits in Doubled-Haploid Population Derived from Texas Wheat Cultivars.

Genes·2025
Same author

Breaking Barriers: A Protocol to Investigate Intestinal Pathogen Adhesion, Invasion, and Translocation Through In Vitro, In Vivo, and Imaging Technologies.

Methods in molecular biology (Clifton, N.J.)·2025

Related Experiment Video

Updated: Aug 14, 2025

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

2.8K

Petri-plate, bacteria, and laser optical scattering sensor.

Arun K Bhunia1,2,3,4, Atul K Singh1,5, Kyle Parker6

  • 1Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, United States.

Frontiers in Cellular and Infection Microbiology
|January 9, 2023
PubMed
Summary

A new optical scattering sensor, BARDOT, identifies bacteria directly on Petri plates. This technology advances microbial diagnostics and research, building upon the foundational Petri plate.

Keywords:
BARDOTPetri-plateRamanbacterial colonyhyperspectral imaginglaseroptical sensorscatter signature

More Related Videos

ScanLag: High-throughput Quantification of Colony Growth and Lag Time
07:47

ScanLag: High-throughput Quantification of Colony Growth and Lag Time

Published on: July 15, 2014

16.3K
Monitoring Spatial Segregation in Surface Colonizing Microbial Populations
07:40

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Published on: October 29, 2016

11.1K

Related Experiment Videos

Last Updated: Aug 14, 2025

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

2.8K
ScanLag: High-throughput Quantification of Colony Growth and Lag Time
07:47

ScanLag: High-throughput Quantification of Colony Growth and Lag Time

Published on: July 15, 2014

16.3K
Monitoring Spatial Segregation in Surface Colonizing Microbial Populations
07:40

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Published on: October 29, 2016

11.1K

Area of Science:

  • Microbiology
  • Biotechnology
  • Biosensing

Background:

  • The Petri plate, invented in 1887, is crucial for isolating, identifying, and studying microorganisms.
  • Traditional microbial identification relies on pure culture isolates, a process that predates advanced gene sequencing.
  • Manipulating microbes on Petri plates aids research in gene expression, virulence, antibiotic resistance, and product development.

Purpose of the Study:

  • To introduce a novel optical scattering sensor for direct bacterial detection on Petri plates.
  • To explore the potential of this technology in revolutionizing microbial diagnostics and research.
  • To provide a tool for applications in clinical and food microbiology laboratories.

Main Methods:

  • Development of an optical scattering sensor named BARDOT (bacterial rapid detection using optical scattering technology).
  • Utilizing a red-diode laser for bacterial identification directly on the Petri plate.
  • Comparing the sensor's capabilities to Raman scattering and hyperspectral imaging systems.

Main Results:

  • BARDOT can identify and study bacteria directly on the Petri plate.
  • This direct detection method offers a new diagnostic tool for microbiology.
  • The technology has potential applications comparable to advanced imaging systems.

Conclusions:

  • Direct detection and identification of bacterial colonies on Petri plates using sensing devices represent a significant advancement.
  • BARDOT technology has the potential to revolutionize microbial research, including gene sequencing, pathogenicity studies, and antibiotic susceptibility testing.
  • This innovation offers a powerful diagnostic tool for clinical and food microbiology, enhancing the study of industrially beneficial traits.