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Updated: Aug 14, 2025
Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field
Published on: August 27, 2021
Protocol for direct measurement of stability and activity of mitochondria electron transport chain complex II
Himangshu S Bose1, Nicole E Doetch2
1Laboratory of Biochemistry and Cell Biology, Department of Biomedical Sciences, Mercer University School of Medicine, Hoskins Research Building 1250 East 66th Street, Savannah, GA 31404, USA; Anderson Cancer Institute, Memorial University Medical Center, Savannah, GA 31404, USA.
Abstract:
Mitochondria electron transport chain (ETC) complex II is essential for steroid metabolism. Here, we present a protocol to measure the stability and activity of mitochondria ETC complex II. We first describe mitochondria isolation from cell lines and tissues. We then detail how to determine the stability of ETC complex II using isothermal calorimetry and quantification of steroidogenesis using activity assays in parallel. Finally, we describe the steps to perform radioimmunoassay (RIA) to confirm the activity of ETC complex II. For complete details on the use and execution of this protocol, please refer to Bose et al. (2020).1.
