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Related Experiment Video

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Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in Rodents
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Remote Ischaemic Pre-Conditioning Reduces Intestinal Ischaemia Reperfusion Injury in a Newborn Rat.

Ian Howard Jones1, Denise Tao2, Bhumita Vagdama2

  • 1Department of Paediatric Surgery and Urology, Southampton Children's Hospital, Southampton, UK; University Surgery Unit, Faculty of Medicine, University of Southampton, Southampton, UK.

Journal of Pediatric Surgery
|January 9, 2023
PubMed
Summary

Remote ischaemic conditioning (RIC) significantly reduced bowel injury in a newborn rat model. This novel therapy shows promise for treating intestinal diseases in infants, decreasing both macroscopic and microscopic damage.

Keywords:
Intestinal ischaemiaNECNecrotising enterocolitisRICRemote ischaemic conditioning

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Area of Science:

  • Neonatal Medicine
  • Surgical Research
  • Gastroenterology

Background:

  • Ischaemia-reperfusion injury (IRI) significantly impacts neonatal bowel pathologies, notably necrotising enterocolitis (NEC).
  • Remote ischaemic conditioning (RIC) is a potential therapeutic strategy for mitigating IRI across various organ systems.

Purpose of the Study:

  • To investigate the therapeutic efficacy of RIC in reducing intestinal injury in a neonatal model.
  • To evaluate RIC's potential as a novel treatment for neonatal intestinal diseases.

Main Methods:

  • An established rat pup intestinal IRI model, mimicking necrotising enterocolitis (NEC), was utilized.
  • Animals were randomized into three groups: IRI only, IRI + RIC, and sham laparotomy.
  • Macroscopic injury, microscopic severity (Chiu-Park scoring), neutrophil infiltration (myeloperoxidase activity), and HIF-1α expression were assessed.

Main Results:

  • RIC significantly decreased macroscopic intestinal injury extent (58% vs. 100% in IRI only group, p=0.003).
  • Microscopic injury scores were significantly lower in the RIC group compared to IRI alone (p=0.014).
  • Intestinal myeloperoxidase activity was reduced with RIC, indicating decreased neutrophil infiltration (p=0.047).

Conclusions:

  • Remote ischaemic conditioning effectively reduces the extent and severity of bowel injury in this neonatal animal model.
  • These findings support RIC as a promising therapeutic candidate for neonatal intestinal diseases.