Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fast Image Segmentation Toward Automation of 3D Ice Printing.

Chemical & biomedical imaging·2026
Same author

A data-driven framework for modeling the dendritic spine continuum using dimensionality reduction and clustering toward understanding synaptic plasticity.

PloS one·2026
Same author

Revisiting the INSPIRE trial: antibody profiling reveals high prevalence of occult autoimmunity.

Respiratory research·2026
Same author

Identification of the seven critical residues that control ZIKV-DENV cross-reactivity to engineer a non-cross-reactive ZIKV vaccine.

Cell reports·2025
Same author

IP-to-MS: An Unbiased Workflow for Antigen Profiling.

Journal of proteome research·2025
Same author

Physics of microscale freeform 3D printing of ice.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: Aug 14, 2025

Mechanostimulation of Multicellular Organisms Through a High-Throughput Microfluidic Compression System
09:56

Mechanostimulation of Multicellular Organisms Through a High-Throughput Microfluidic Compression System

Published on: December 23, 2022

1.7K

Mechanostimulation of Multicellular Organisms Through a High-Throughput Microfluidic Compression System.

Utku M Sönmez1, Nolan Frey2, Jonathan S Minden3

  • 1Department of Mechanical Engineering, Carnegie Mellon University.

Journal of Visualized Experiments : Jove
|January 9, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel microfluidic system for precise mechanical stimulation of Drosophila melanogaster embryos. This high-throughput device enables simultaneous compression and imaging of hundreds of embryos, advancing developmental biology research.

More Related Videos

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans
10:39

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Published on: February 19, 2018

10.8K
A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression
07:23

A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression

Published on: September 13, 2019

6.7K

Related Experiment Videos

Last Updated: Aug 14, 2025

Mechanostimulation of Multicellular Organisms Through a High-Throughput Microfluidic Compression System
09:56

Mechanostimulation of Multicellular Organisms Through a High-Throughput Microfluidic Compression System

Published on: December 23, 2022

1.7K
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans
10:39

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Published on: February 19, 2018

10.8K
A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression
07:23

A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression

Published on: September 13, 2019

6.7K

Area of Science:

  • Developmental Biology
  • Biophysics
  • Microfluidics

Background:

  • Embryogenesis involves cell movements generating mechanical forces crucial for gene regulation.
  • Traditional methods for mechanical embryo stimulation (aspiration, coverslip compression) are imprecise and low-throughput.

Purpose of the Study:

  • To develop a microfluidic system for precise, high-throughput mechanical stimulation of Drosophila melanogaster embryos.
  • To enable automated embryo alignment, immobilization, compression, and collection for advanced analyses.

Main Methods:

  • A microfluidic system with pneumatically actuated deformable sidewalls was fabricated on a glass coverslip.
  • The system features parallelized microchannels allowing simultaneous stimulation of hundreds of embryos.
  • Biocompatible materials (PDMS) and fluid flow enable long-term, media-dependent experiments.

Main Results:

  • The system precisely compresses whole Drosophila embryos with automated alignment and immobilization.
  • Parallelized lanes allow simultaneous mechanical stimulation of hundreds of embryos.
  • Facilitates high-resolution microscopy and enables rapid sample collection for downstream -omics assays.

Conclusions:

  • This microfluidic system offers a precise, automated, and high-throughput method for studying mechanical forces in embryogenesis.
  • The scalable design benefits various biological systems requiring mechanical stimulation, immobilization, or automated alignment.
  • Enables advanced post-stimulation analyses previously unattainable with traditional methods.