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Updated: Aug 14, 2025
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
A label-free fluorescent aptasensor based on a novel exponential rolling circle amplification for highly sensitive
Daozhong Zhu1, Ting Huang2, Qianying Zhou2
1NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, PR China; Guangzhou Customs Technology Center, People's Republic of China, Guangzhou 510623, PR China.
A new aptasensor uses exponential rolling circle amplification (ERCA) for highly sensitive, label-free detection of ochratoxin A (OTA). This method offers a sensitive tool for food safety analysis.
Area of Science:
- Analytical Chemistry
- Biotechnology
Background:
- Ochratoxin A (OTA) is a mycotoxin with significant implications for food safety.
- Rapid and sensitive detection methods for OTA are crucial for regulatory compliance and public health.
Purpose of the Study:
- To develop a novel aptasensor for ultrasensitive, label-free fluorescence detection of OTA.
- To utilize exponential rolling circle amplification (ERCA) for enhanced signal generation.
Main Methods:
- An aptasensor was designed incorporating ERCA for OTA detection.
- OTA binding to its aptamer triggered a cascade reaction initiating RCA.
- Recycled RCA and G4 dimer/ThT fluorescence were employed for signal amplification.
Main Results:
- The aptasensor demonstrated a wide linear range for OTA detection (10 fg/mL to 10 ng/mL).
- An ultra-low detection limit of 4.3 fg/mL was achieved.
- Satisfactory recovery rates were observed in real sample analyses.
Conclusions:
- The developed ERCA-based aptasensor provides a highly sensitive and efficient method for OTA detection.
- This technology holds significant promise for applications in food safety and medical diagnostics.
