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Related Experiment Videos

Penicillin tolerance in Streptococcus faecium ATCC 9790.

I Said1, H Fletcher, A Volpe

  • 1Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.

Antimicrobial Agents and Chemotherapy
|July 1, 1987
PubMed
Summary

Tolerant Streptococcus faecium strains show higher muramidase 2 and lower trypsin-activable muramidase 1 levels. This difference impacts bacterial lysis rates, especially in the presence of antibiotics.

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Area of Science:

  • Microbiology
  • Bacterial Physiology

Background:

  • Streptococcus faecium is an opportunistic pathogen.
  • Bacterial cell wall lysis is a key target for antibiotics.
  • Muramidases are enzymes involved in cell wall remodeling.

Purpose of the Study:

  • To investigate the role of muramidases in Streptococcus faecium antibiotic tolerance.
  • To compare enzymatic profiles of tolerant and susceptible strains.

Main Methods:

  • Quantification of muramidase 2 and trypsin-activable muramidase 1 levels.
  • Assessment of bacterial lysis rates in buffer and with antibiotics.
  • Evaluation of lysis rates with Triton X-100 addition.

Main Results:

  • Tolerant S. faecium strains exhibited higher muramidase 2 and lower trypsin-activable muramidase 1.

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  • Susceptible strains lysed more rapidly than tolerant strains under certain conditions.
  • Triton X-100 normalized lysis rates between susceptible and tolerant strains.

    • Conclusions:

    • Muramidase levels are associated with antibiotic tolerance in S. faecium.
    • Enzymatic differences influence bacterial susceptibility to lysis.
    • Membrane integrity, potentially affected by muramidases, plays a role in tolerance.