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Related Concept Videos

The Parathyroid Glands00:59

The Parathyroid Glands

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The two pairs of parathyroid glands embedded within the posterior surface of the thyroid gland are restricted by a dense capsule around them. These glands comprise two distinct cell populations—parathyroid oxyphil and parathyroid principal cells- pivotal in calcium homeostasis.
Oxyphil cells, whose functions remain elusive, emerge during late puberty, adding a layer of complexity to the parathyroid gland's intricacies. In contrast, principal parathyroid cells undertake a vital role by...
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Hormones and Bone Tissue01:17

Hormones and Bone Tissue

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The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
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Functions of Thyroid Hormones01:18

Functions of Thyroid Hormones

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The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
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Roles of Electrolytes: Calcium and Phosphate01:27

Roles of Electrolytes: Calcium and Phosphate

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Calcium and phosphate are essential electrolytes in the human body, with calcium being the most abundant mineral. Around 99% of the body's calcium is stored in the skeleton and teeth, forming a crystal lattice of mineral salts in combination with phosphates. Calcium plays crucial roles in various bodily functions such as blood clotting, neurotransmitter release, muscle tone maintenance, and nervous and muscle tissue excitability.
The calcium concentration in blood plasma is primarily...
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Synthesis and Functions of Calcitonin00:51

Synthesis and Functions of Calcitonin

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Calcitonin, a vital polypeptide hormone, regulates calcium levels within body fluids. It is released by the parafollicular cells, also known as C cells, situated in the follicular epithelium of the thyroid gland. Calcitonin responds to fluctuations in blood calcium levels and the influence of gastrointestinal hormones like gastrin and cholecystokinin.
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...
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Related Experiment Video

Updated: Aug 14, 2025

Generation of Hypoparathyroid Rats via Carbon-Nanoparticle-Assisted Parathyroidectomy
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Parathyroid hormone reference intervals in adults using second- and third-generation assays.

Stine Linding Andersen1,2, Jesper Karmisholt2,3, Peter Vestergaard2,3,4

  • 1Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark.

Scandinavian Journal of Clinical and Laboratory Investigation
|January 10, 2023
PubMed
Summary
This summary is machine-generated.

Second- and third-generation parathyroid hormone (PTH) assays yield non-interchangeable results. Establishing method-specific reference ranges is crucial for accurate clinical interpretation following assay changes.

Keywords:
PTHhyperparathyroidismhypoparathyroidismimmunoassayvitamin D

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Last Updated: Aug 14, 2025

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Area of Science:

  • Clinical Biochemistry
  • Analytical Chemistry
  • Endocrinology

Background:

  • Parathyroid hormone (PTH) assays are routine biochemical tests.
  • Different generations of PTH assays (second- and third-generation) exist.
  • Understanding assay-specific performance is vital for clinical practice.

Purpose of the Study:

  • To compare PTH levels between second- and third-generation assays.
  • To establish method-specific reference intervals for PTH assays.
  • To inform clinical practice regarding PTH assay transitions.

Main Methods:

  • Method comparison using Passing-Bablok regression analysis.
  • Establishment of reference intervals using adult blood donor samples.
  • Utilized third-generation (Cobas 8000, Roche Diagnostics) and second-generation (Atellica, Siemens; Alinity, Abbott) PTH assays.

Main Results:

  • Third-generation assay showed lower PTH levels compared to second-generation assays, with varying differences based on concentration.
  • Method-specific reference intervals were established: 1.8-8.5 pmol/L (Siemens), 2.4-10.9 pmol/L (Abbott), and 1.8-7.0 pmol/L (Roche).
  • Samples with low 25-hydroxy-vitamin D (<50 nmol/L) were excluded from reference interval calculations.

Conclusions:

  • Second- and third-generation PTH assays are not interchangeable.
  • Laboratories must inform clinicians about assay changes.
  • Method-specific reference ranges are essential for accurate PTH testing and patient care.