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Related Experiment Video

Updated: Aug 14, 2025

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
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Microfluidically-generated Encapsulated Spheroids (μ-GELS): An All-Aqueous Droplet Microfluidics Platform for

Jennifer Kieda1,2,3, Sila Appak-Baskoy2,3,4, Morteza Jeyhani2,3,5

  • 1Graduate Program in Biomedical Engineering, Toronto Metropolitan University, TorontoM5B 2K3, Canada.

ACS Biomaterials Science & Engineering
|January 10, 2023
PubMed
Summary

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This summary is machine-generated.

This study introduces an all-aqueous droplet microfluidic platform for creating multicellular spheroids (MCSs) without cytotoxic solvents. The system enables on-chip gelation and demonstrates viability for cancer drug testing.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Spheroids are vital in vitro tumor models, but current methods often use toxic organic solvents.
  • Existing all-aqueous methods struggle with complete on-chip hydrogel gelation.

Purpose of the Study:

  • To develop an enhanced droplet microfluidic platform for all-aqueous hydrogel multicellular spheroid (MCS) formation.
  • To enable on-chip gelation of MCSs without cytotoxic organic solvents.
  • To validate the platform for cell viability and cancer drug testing.

Main Methods:

  • Utilized a droplet microfluidic system to generate dextran-alginate droplets with MCF-7 breast cancer cells.
  • Incorporated a polyethylene glycol continuous phase and a calcium chloride crosslinking solution.
Keywords:
aqueous two-phase systemdroplet microfluidicshydrogelmicrogelspheroid

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  • Employed an embedded capillary at a second junction to delay gelation and prevent channel clogging.

    • Main Results:

    • Achieved on-chip gelation of all-aqueous hydrogel MCSs.
    • Demonstrated MCS viability for up to 6 days in drug-free conditions.
    • Confirmed the platform's utility for cancer drug screening with doxorubicin, showing dose-dependent cell death.

    Conclusions:

    • The enhanced droplet microfluidic platform successfully forms viable, all-aqueous hydrogel MCSs on-chip.
    • This method offers a non-cytotoxic alternative for spheroid generation and cancer drug testing.
    • The system holds promise for improved in vitro tumor modeling and pharmaceutical screening.