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Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
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Part I: Non-faradaic electrochemical impedance-based DNA biosensor for detecting phytopathogen - Ralstonia

Rhea Patel1, Madhuri Vinchurkar2, Aatha Mohin Shaikh2

  • 1Center for Research in Nanotechnology and Science, Indian Institute of Technology Bombay, Mumbai 400076, India.

Bioelectrochemistry (Amsterdam, Netherlands)
|January 11, 2023
PubMed
Summary

We developed a highly sensitive, label-free DNA sensor using gold electrodes to detect the agricultural pathogen Ralstonia solanacearum. This impedimetric sensor achieves low detection limits for DNA hybridization, aiding in early disease identification.

Keywords:
AgricultureDNA hybridizationInterdigitated electrodeNon-faradaic impedance spectroscopyPhytopathogen detection

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Area of Science:

  • Biosensor technology
  • Electrochemical sensing
  • Agricultural microbiology

Background:

  • Ralstonia solanacearum is a devastating soil-borne pathogen affecting numerous crops.
  • Early and accurate detection of R. solanacearum is crucial for effective disease management in agriculture.
  • Existing detection methods can be time-consuming or require complex laboratory equipment.

Purpose of the Study:

  • To develop a novel, label-free impedimetric DNA sensor for sensitive detection of Ralstonia solanacearum.
  • To utilize micro-sized gold interdigitated electrodes (IDE) for enhanced sensor performance.
  • To establish a rapid and reliable method for identifying this agricultural pathogen.

Main Methods:

  • Immobilization of a specific single-stranded DNA (ssDNA) probe (lpxC4) onto gold IDE surfaces.
  • Validation of probe immobilization using contact angle and ATR-FTIR analysis.
  • Detection of DNA target hybridization via non-faradaic electrochemical impedance spectroscopy (EIS).

Main Results:

  • The developed sensor demonstrated high sensitivity, detecting DNA target concentrations as low as 0.1 ng/µL.
  • Successful hybridization detection of R. solanacearum DNA from infected eggplant samples.
  • Cross-reactivity studies confirmed the specificity of the DNA probe.

Conclusions:

  • A label-free, non-faradaic impedimetric DNA sensor was successfully developed for R. solanacearum detection.
  • The sensor utilizes micro-sized gold IDEs and achieves ultra-low sample volume detection.
  • This work provides a foundation for developing field-deployable sensors for plant pathogen identification.