Drawbacks in the efficient monitoring of gold nanoparticle-based cisplatin delivery systems formation by HPLC-ICP-MS
Anna M Wróblewska1, Nina Gos1, Joanna Zajda1
1Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego St. 3, 00-664 Warsaw, Poland.
Abstract:
Since chemotherapy suffers many limitations related to side effects of anticancer drugs (e.g. cisplatin - CDDP), nanoparticles are probed as carriers in targeted drug delivery. Gold nanoparticles (AuNPs) are broadly investigated due to their biocompatibility, nontoxicity, and tunable surface. Despite many AuNPs-cisplatin systems (AuNP-CS) reports found in the literature, only a few include studies of their synthesis and formation efficiency using analytical tools providing simultaneously qualitative and quantitative analytical information. Therefore, this research continues our previous study of AuNP-CS formation investigated by capillary electrophoresis with inductively coupled plasma mass spectrometry (ICP-MS). Namely, it presents the analogical approach but employs the coupling of another separation technique: isocratic reversed-phase high-performance liquid chromatography. The study concerns the difficulties of analytical method optimization path and contains a discussion of the observed problematic issues related to the analysis and preparation of AuNP-CS. Moreover, the presented work confronts the performance and applicability of both tools for the scrutiny of AuNP-CS, especially considering the comparison of their resolution power.
Insights
This study compares two analytical methods, capillary electrophoresis and high-performance liquid chromatography, for analyzing gold nanoparticle-cisplatin (AuNP-CS) drug delivery systems. Both methods were evaluated for their effectiveness in characterizing AuNP-CS formation and efficiency.
Area of Science:
- Analytical Chemistry
- Materials Science
- Nanotechnology
Background:
- Chemotherapy faces limitations due to side effects of anticancer drugs like cisplatin.
- Nanoparticles, particularly gold nanoparticles (AuNPs), are explored for targeted drug delivery due to their biocompatibility and tunable properties.
- Characterizing the synthesis and formation efficiency of gold nanoparticle-cisplatin systems (AuNP-CS) requires robust analytical techniques.
Purpose of the Study:
- To compare the analytical performance of capillary electrophoresis with inductively coupled plasma mass spectrometry (CE-ICP-MS) and isocratic reversed-phase high-performance liquid chromatography (RP-HPLC) for AuNP-CS analysis.
- To investigate the challenges and optimization pathways for analytical methods applied to AuNP-CS.
- To evaluate the resolution power and applicability of both CE-ICP-MS and RP-HPLC for scrutinizing AuNP-CS.
Main Methods:
- Synthesis and preparation of gold nanoparticle-cisplatin (AuNP-CS) systems.
- Analysis using capillary electrophoresis coupled with inductively coupled plasma mass spectrometry (CE-ICP-MS).
- Analysis using isocratic reversed-phase high-performance liquid chromatography (RP-HPLC).
Main Results:
- Both CE-ICP-MS and RP-HPLC were employed to analyze AuNP-CS formation.
- The study details the difficulties encountered during analytical method optimization.
- A comparative discussion on the performance, applicability, and resolution power of both analytical techniques for AuNP-CS scrutiny is presented.
Conclusions:
- This research provides insights into the analytical challenges associated with AuNP-CS characterization.
- The study offers a comparative analysis of CE-ICP-MS and RP-HPLC for evaluating nanoparticle-drug complex formation.
- The findings contribute to the understanding of analytical tools for quality control in nanoparticle-based drug delivery systems.
