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A binary interaction map between turnip mosaic virus and Arabidopsis thaliana proteomes
Fernando Martínez1, José L Carrasco1, Christina Toft1
1Instituto de Biología Integrativa de Sistemas (I2SysBio), CSIC - Universitat de València, 46980, Paterna, Spain.
Communications Biology
|January 11, 2023
Summary
This study maps protein interactions between turnip mosaic virus (TuMV) and Arabidopsis thaliana, revealing the viral RNA-dependent RNA polymerase NIb targets key host regulators. These findings illuminate virus-host dynamics and potential control strategies.
Area of Science:
- Plant Virology
- Molecular Plant-Microbe Interactions
- Systems Biology
Background:
- Viruses, as obligate intracellular parasites, engage in complex co-evolutionary relationships with their hosts, characterized by intricate protein-protein interactions.
- Understanding these interactions is crucial for deciphering viral pathogenesis and host defense mechanisms.
Purpose of the Study:
- To identify and characterize novel protein-protein interactions between turnip mosaic virus (TuMV) and its host, Arabidopsis thaliana.
- To construct and analyze the TuMV-Arabidopsis thaliana interaction network.
- To investigate the functional roles of host proteins involved in viral interactions.
Main Methods:
- High-throughput yeast two-hybrid screening to identify viral-host protein interactions.
- In planta validation of selected interactions using bimolecular fluorescence complementation (BiFC) assays.
- Network construction and analysis, including intravirus and intrahost connections.
- Functional characterization of host proteins in TuMV infection dynamics using mutant plants.
Main Results:
- Identified 378 novel protein-protein interactions between TuMV and Arabidopsis thaliana.
- The viral RNA-dependent RNA polymerase NIb showed the highest number of interactions, including with salicylic acid-dependent transcription regulators.
- Constructed a network of 399 TuMV-Arabidopsis thaliana interactions, revealing targeted host proteins are highly connected, expressed, and involved in plant defense.
- Validated a proviral role for the transcriptional regulator TGA1 in TuMV infection.
Conclusions:
- The study provides a comprehensive map of TuMV-Arabidopsis thaliana protein interactions, highlighting the virus's manipulation of host cellular machinery.
- Targeted host proteins possess characteristics suggesting their importance in host defense and viral propagation.
- Comparative analysis with animal viruses suggests conserved fundamental mechanisms in virus-host interactions across kingdoms.
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