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Updated: Aug 14, 2025

Enzymatic Isolation of Skeletal Muscle Interstitial Extracellular Vesicles
Published on: February 7, 2025
Exosome-mediated regulatory mechanisms in skeletal muscle: a narrative review
Zhaolu Wang, Jinjin Yang1, Xiaohui Sun1
1College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Abstract:
Skeletal muscle plays a paramount role in physical activity, metabolism, and energy balance, while its homeostasis is being challenged by multiple unfavorable factors such as injury, aging, or obesity. Exosomes, a subset of extracellular vesicles, are now recognized as essential mediators of intercellular communication, holding great clinical potential in the treatment of skeletal muscle diseases. Herein, we outline the recent research progress in exosomal isolation, characterization, and mechanism of action, and emphatically discuss current advances in exosomes derived from multiple organs and tissues, and engineered exosomes regarding the regulation of physiological and pathological development of skeletal muscle. These remarkable advances expand our understanding of myogenesis and muscle diseases. Meanwhile, the engineered exosome, as an endogenous nanocarrier combined with advanced design methodologies of biomolecules, will help to open up innovative therapeutic perspectives for the treatment of muscle diseases.
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