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Portable light-sheet optofluidic microscopy for 3D fluorescence imaging flow cytometry.

Jeonghwan Son1, Biagio Mandracchia1, Aaron D Silva Trenkle1

  • 1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA. shu.jia@gatech.edu.

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|January 12, 2023
PubMed
Summary
This summary is machine-generated.

Portable light-sheet optofluidic microscopy (PLSOM) enables high-throughput 3D fluorescence cytometric imaging. This compact, low-cost system offers subcellular resolution and compatibility with microfluidics for advanced cell analysis.

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Area of Science:

  • Biomedical Engineering
  • Optical Microscopy
  • Cell Biology

Background:

  • Imaging flow cytometry (IFC) integrates flow cytometry with microscopy for single-cell analysis.
  • Existing 3D IFC systems face challenges with complexity and microfluidic compatibility.

Purpose of the Study:

  • To develop a portable, user-friendly system for 3D fluorescence cytometric imaging.
  • To overcome limitations of current 3D IFC instrumentation.

Main Methods:

  • Development of portable light-sheet optofluidic microscopy (PLSOM).
  • Utilized a compact, open-top light-sheet configuration compatible with standard microfluidic chips.
  • Demonstrated 3D IFC using phantom and cell samples.

Main Results:

  • PLSOM achieves subcellular resolution (2-4 μm) in 3D.
  • High throughput of approximately 1000 cells per second.
  • The system is portable (30x10x26 cm) and low-cost.

Conclusions:

  • PLSOM provides a versatile platform for 3D flow cytometric imaging.
  • The system's adaptability facilitates broad adoption in cell analysis research.
  • PLSOM offers a practical solution for advanced, high-throughput 3D cell imaging.