Controlled Differentiation of Human Neural Progenitor Cells on Molybdenum Disulfide/Graphene Oxide Heterojunction...

Ultrathin MoS₂-MoO_3- heterojunction nanosheets with unique features were introduced as biocompatible, non-cytotoxic, and visible light-sensitive stimulator layers for the controlled differentiation of human neural progenitor cells (hNPCs) into nervous lineages. hNPC differentiation was also investigated on reduced graphene oxide (rGO)-containing scaffolds, that is, rGO and rGO/MoS₂-MoO_3- nanosheets. In darkness, hNPC differentiation into neurons increased on MoS₂-MoO_3- by a factor of 2.7 due to the excellent biophysical cues and further increased on rGO/MoS₂-MoO_3- by a factor of 4.4 due to a synergistic effect induced by the rGO. The MoO_3- domains with antioxidant activity and LSPR absorption induced p-type doping in MoS₂-MoO_3-. Under photostimulation, the hNPCs on the MoS₂-MoO_3- exhibited higher differentiation into glial cells by a factor of 1.4, and the decrease in photo-electron current to hNPCs due to the induction of more p-type doping in the MoS₂-MoO_3-. While the increase in neuronal differentiation of hNPCs on rGO/MoS₂-MoO_3- by a factor of 1.8 was ascribed to the presence of rGO as an ultrafast electron transferor which quickly transferred photogenerated electrons to hNPCs before their transfer to free radicals, these results demonstrated the promising potential of MoS₂-based scaffolds for applying in the controllable repair and/or regeneration of diseases/disorders related to the nervous system.