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Updated: Aug 14, 2025

Generation of a Human iPSC-Based Blood-Brain Barrier Chip

Generation of a Human iPSC-Based Blood-Brain Barrier Chip

Published on: March 2, 2020

Engineering Human Brain Assembloids by Microfluidics.

Yujuan Zhu^1,2, Xiaoxuan Zhang¹, Lingyu Sun¹

¹Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.

Advanced Materials (Deerfield Beach, Fla.)

|January 12, 2023

Summary

This summary is machine-generated.

Researchers developed a microfluidic system to create patterned human brain assembloids. This flexible technology enables scalable production of brain organoids for disease modeling and drug discovery.

Keywords:

assembloid-on-a-chip brain assembloids brain organoids microcapsules microfluidics

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Area of Science:

Neuroscience
Biotechnology
Bioengineering

Background:

Brain assembloids are valuable for modeling human brain development and disease.
Conventional methods face challenges in producing uniform and flexibly assembled brain organoids.

Purpose of the Study:

To present an engineered strategy for generating human brain assembloids with desired patterning using microfluidic technology.
To enable flexible, scalable, and controlled assembly of brain organoids.

Main Methods:

Human induced pluripotent stem cells encapsulated in microcapsules via microfluidic electrospray to form brain region-specific organoids.
Organoids assembled in a microfluidic chip with micropillar and microhole arrays for controlled fusion.
Creation of patterned brain assembloids using designed 1D sequences or 2D arrays of organoid microcapsules.

Main Results:

Efficient formation of brain region-specific organoids.
Successful assembly and fusion of organoids into patterned brain assembloids.
Demonstrated growth and function of assembloids composed of cortical, hippocampal, and thalamic organoids, showing active neural migration and interaction.

Conclusions:

The microfluidic system provides a flexible, scalable, and controlled method for producing patterned human brain assembloids.
This technology holds significant potential for applications in neurological and biomedical fields, including disease modeling, regenerative medicine, and drug discovery.