Related Experiment Video
Updated: Aug 14, 2025
Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
Published on: March 22, 2020
A sensitive fluorescent assay based on gold-nanoclusters coated on molecularly imprinted covalent organic frameworks
Lehan Zhao1, Xianfeng Lin1, Nuo Duan2
1State Key Laboratory of Food Science and Technology, School of Food Science and Technology, International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.
Abstract:
Malachite green (MG), as a parasiticide, is widely used in aquaculture to increase the production of the fishery industry. It poses a great danger to both the food system and the human body. In this study, a one-pot reverse microemulsion polymerization was employed to combine the gold nanoclusters (AuNCs) with molecularly imprinted polymers (MIPs) and covalent organic frameworks (COFs) to synthesize an efficient fluorescent hybrid probe (AuNCs@COFs@MIPs) for selective detection of MG. The specific recognition of AuNCs@COFs@MIPs towards MG triggers the fluorescence quenching of AuNCs. The fluorescent response was linearly related to the concentration over the range of 10-150 nmol/L with a limit of detection of 2.78 nmol/L. In addition, the proposed probe was further applied to fish and water samples. A favorable recovery ranged from 97.34 to 101.51 % toward trace amounts of MG indicating its promising application for detecting residue of veterinary drugs.
