Use of exogenous substrate in Chlorella cultivation: Strategy for biomass and polyhydroxybutyrate production

The aim of this study was to investigate the influence of exogenous carbon supplementation and nitrogen source reduction on Chlorella fusca LEB 111 growth, biomass composition, and polyhydroxybutyrate accumulation. First, assays were performed with 50 % and 25 % reduced nitrogen source concentrations (NaNO₃). In the second stage, the influence of culture supplementation with 10, 20, and 30 mg L^-1 D-xylose, associated with 50 and 25 % reductions in NaNO₃, was evaluated. The experiments conducted with a 25 % reduction in NaNO₃ and supplementation with 10 mg L^-1 D-xylose resulted in a positive effect on the biomass productivity of C. fusca LEB 111, with production as high as 354.4 mg L^-1 d^-1. The maximum concentration of PHB extracted from C. fusca LEB 111 was 3.7 % (w w^-1) and was obtained when the microalgae were cultivated with a 25 % of reduction in NaNO₃ and supplementation of D-xylose at 20 mg L^-1. Therefore, this study brings new perspectives regarding reducing the use of nutritional sources and using exogenous carbon sources in using microalgae to produce molecules of high biotechnological potential.