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RBM4 regulates cellular senescence via miR1244/SERPINE1 axis.

Luning Wang1, Xiaohong Zhang2, Junxiu Sheng3

  • 1Institute of Cancer Stem Cell, Dalian Medical University, Dalian, 116044, China.

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|January 13, 2023
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Summary
This summary is machine-generated.

The RNA binding protein RBM4 promotes cancer progression by inhibiting cellular senescence. Depleting RBM4 induces senescence and suppresses tumor growth, revealing RBM4 as a potential cancer therapeutic target.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Cellular senescence is a key tumor suppressor mechanism.
  • RNA-binding proteins (RBPs) influence cancer progression, but their role in senescence is unclear.

Purpose of the Study:

  • To investigate the role of RNA binding protein RBM4 in regulating cellular senescence and cancer progression.

Main Methods:

  • Depletion of RBM4 in various cell types, including cancer cells.
  • Analysis of SERPINE1 and miR-1244 expression levels.
  • In vitro and in vivo cancer progression assays.
  • Assessment of senescence induction using SERPINE1 inhibitors and miR-1244 mimics.

Main Results:

  • RBM4 depletion induced cellular senescence and inhibited cancer cell proliferation in vitro and in vivo.
  • RBM4 knockdown increased SERPINE1 expression by reducing miR-1244 levels.
  • miR-1244 directly targets SERPINE1, and RBM4 regulates miR-1244 stability.
  • Inhibition of SERPINE1 or mimicry of miR-1244 reversed RBM4 depletion-induced senescence.

Conclusions:

  • RBM4 regulates cancer progression by controlling cellular senescence through the miR-1244/SERPINE1 axis.
  • This study uncovers a novel mechanism linking RBM4 to senescence.
  • RBM4 represents a potential therapeutic target for cancer treatment.