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Updated: Aug 14, 2025

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
Published on: January 25, 2020
Elucidating the structure and function of the nucleus-The NIH Common Fund 4D Nucleome program
Ananda L Roy1, Richard S Conroy1, Veronica G Taylor1
1Office of Strategic Coordination, National Institutes of Health, Bethesda, MD 20892, USA; Division of Program Coordination, Planning, and Strategic Initiative, National Institutes of Health, Bethesda, MD 20892, USA; Office of the National Institutes of Health Director, National Institutes of Health, Bethesda, MD 20892, USA.
Abstract:
Genomic architecture appears to play crucial roles in health and a variety of diseases. How nuclear structures reorganize over different timescales is elusive, partly because the tools needed to probe and perturb them are not as advanced as needed by the field. To fill this gap, the National Institutes of Health Common Fund started a program in 2015, called the 4D Nucleome (4DN), with the goal of developing and ultimately applying technologies to interrogate the structure and function of nuclear organization in space and time.
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