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3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
Published on: January 25, 2020
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Elucidating the structure and function of the nucleus-The NIH Common Fund 4D Nucleome program.
Ananda L Roy1, Richard S Conroy1, Veronica G Taylor1
1Office of Strategic Coordination, National Institutes of Health, Bethesda, MD 20892, USA; Division of Program Coordination, Planning, and Strategic Initiative, National Institutes of Health, Bethesda, MD 20892, USA; Office of the National Institutes of Health Director, National Institutes of Health, Bethesda, MD 20892, USA.
Molecular Cell
|January 14, 2023
Summary
Understanding genomic architecture is key to health and disease. The 4D Nucleome (4DN) program develops advanced technologies to study nuclear organization over time and space.
Area of Science:
- Genomics
- Cell Biology
- Biophysics
Background:
- Genomic architecture influences health and disease.
- Understanding nuclear structure dynamics is limited by current technology.
Purpose of the Study:
- To develop and apply novel technologies for interrogating nuclear organization.
- To investigate the structure and function of nuclear organization across space and time.
Main Methods:
- The 4D Nucleome (4DN) program initiated in 2015.
- Focus on developing advanced tools for probing and perturbing nuclear structures.
Main Results:
- Technologies are being developed to address limitations in studying nuclear organization.
- The program aims to provide a comprehensive understanding of nuclear architecture dynamics.
Conclusions:
- Advancing technology is crucial for understanding the role of genomic architecture in biological processes.
- The 4D Nucleome program is poised to significantly enhance our knowledge of nuclear organization.
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